Nondestructive Methods for Monitoring Cell Removal During Rat Liver Decellularization
نویسندگان
چکیده
منابع مشابه
Nondestructive nanostraw intracellular sampling for longitudinal cell monitoring.
Here, we report a method for time-resolved, longitudinal extraction and quantitative measurement of intracellular proteins and mRNA from a variety of cell types. Cytosolic contents were repeatedly sampled from the same cell or population of cells for more than 5 d through a cell-culture substrate, incorporating hollow 150-nm-diameter nanostraws (NS) within a defined sampling region. Once extrac...
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INTRODUCTION Liver Fibrosis is the 12th most leading cause of the death in the United States and 45% of all deaths in the developing world are caused by a fibrotic disease. [1] With the increase rate in fibrotic disease, there is an increased need for transplants which is exceeding the number of donors available. Fibrosis in the liver remains silent until it reaches the stage of liver failure, ...
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ژورنال
عنوان ژورنال: Tissue Engineering Part C: Methods
سال: 2016
ISSN: 1937-3384,1937-3392
DOI: 10.1089/ten.tec.2015.0571